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MICROSCOPY

Micro Vice Sample Holder for Microscopes
Mini Plane Sampling Knife
Diamond Windows for Microscopy
EZ Pick Micro Manipulator Tool for Microscopy
Diamond EX-Press Compression Cell
Fluorescent Filters and Filter Sets


1. Micro Vice Sample Holder for Microscopes

Technical information
This accessory with adjustable vice grip allows various shapes and sizes of samples to be held either in compression or in tension for analysis in different microscope makes and models. Samples can be stretched and rotated by well-defined amount and increments. Sample sizes up to 40mm diameter can be accommodated.

Customised stages for Nicolet, Spectra-Tech, Bruker, Biorad, Nikon, Olympus and Renishaw microscope models are available.

Ordering information
P/N J035     Micro Vice Sample Holder


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2. Mini Plane Sampling Knife

Technical information
The patented Mini Plane micro sampling knife enables easy sampling of materials such as polymers, coatings, organic tissues, inorganic objects, fibres and coatings for microscopy analysis. Samples can be scraped or sliced to uniform controlled thickness and depth with minimum damage to specimen. The resultant sample sizes are extremely reproducible. Thickness and depth variation are achieved by precise screw adjustment on the micro plane that moves the cutting blade.

Two types of blades are available.
- A single Sumi-Crystal Synthetic Type IIa Diamond for planning and cutting hard polymers, inorganic materials and fibres or coatings from metallic surfaces. It is also ideal for cutting multi-layer polymers.
- A Carbide Steel blade best for planning and cutting off thin sections of most polymers, coated materials and organic tissues.

Ordering information
P/N J040
P/N J041
Mini Plane Sampling Knife with Carbide Steel Blade
Mini Plane Sampling Knife with Single Crystal Diamond Blade
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3. Diamond Windows for Microscopy

Technical information
These are made of high quality Type IIa Synthetic Diamond with no impurities that will interfere with sample measurements in the UV, visible or infrared regions. The diamond has high throughput over a wide wavelength range, highly resistant to chemical attack, can withstand extreme pressures and easily cleaned. Two different size diameters (2.5mm and 3.5mm) are securely bonded into a 1" x 3" slide plate for use in various microscopes.

Ordering information
P/N J045
P/N J046
Diamond Window 2.5mm diameter in 1" x 3" microscope slide plate
Diamond Window 3.5mm diameter in 1" x 3" microscope slide plate
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4. EZ Pick Micro Manipulator Tool for Microscopy

Technical information
This unique and patented sample manipulation tool is designed to fix directly onto the objective of most microscopes. Manual handling of micro samples is often too unsteady and tedious while the use of joystick controlled micromanipulators can be complex and expensive. The EZ Pick tool is ideal for any application requiring complex sample manipulation as well as quick and easy handling of simple procedures with high throughput benefit.

This highly popular microscope accessory allows precise control of samples directly under the microscope including force application on sample specimen, direction and positioning operations with repeatable accuracy. The EZ Pick Manipulator is supplied equipped with a full range of sampling tools including knives, needles, micro plane and micro sample dislodger. The assembled accessory mounts directly on the objective of the microscope. The tip of the selected sampling tool and the sample specimen on the stage are aligned and brought into focus and by using the x-y-z movements of the microscope stage, the sample can be manipulated, cut, sectioned or operated as required.

4.1 Micro sampling blade knife - The knife holder and blade can be inserted into the EZ Pick tool for cutting micro specimens from samples in any direction without rotating the sample. Proper tension for cutting the sample is obtained from the spring set on the support post of the EZ Pick. The blades are available in three cutting angle levels of 0, 15 and 30 degrees all fitting into one holder.

4.2 Diamond micro plane - The plane mounts directly in the EZ Pick tool and is used for planning or scraping off the surfaces of samples. The planed surface fragment can then be collected for analysis or the exposed surface layer of the bulk material can then be analysed The cutting surface of the micro plane is a single crystal diamond set into the tip of the blade and can cut thickness from 1-2 microns up to 50 microns.

4.3 Standard Tool Kit - The complete standard tool kit comprises of the following:
1 offEZ Pick Tool
3 offElectrically polished Tungsten Needles (1" long x 0.5mm diameter each with 1, 2.5 and 5 micron tip)
2 offNeedle Holders (pin vice style)
1 offNeedle Positioner used for initial focusing of needles in the EZ Pick tool
1 offMicro Sample Dislodger used for dislodging the selected micro specimen from the needle onto a sample plate for analysis
2 offPositioning Rings
2 offAdjusting Wrenches (Allen Keys)

4.4 Four versions of the EZ Pick are available. Together, the EZ Pick covers most optical microscopes (including IR, Raman, Fluorescence and Stereoscopes) A working distance of at least 5mm and an x-y-z microscope stage are required. The following options are available:
EZ Pick Standard accommodates objectives with diameters of 18-32mm
EZ Pick - 40 accommodates objectives with diameters 27-40mm
EZ Pick - 63 accommodates objectives with diameters 54-63mm
EZ Pick - PE a special version for Perkin Elmer FT-IR microscopes.

Ordering information
P/N J050EZ Micro Manipulator - Standard Tool kit
P/N J051EZ Micro Manipulator - 40 Tool Kit
P/N J052EZ Micro Manipulator - 60 Tool kit
P/N J053EZ Micro Manipulator - PE Tool Kit
P/N J054EZ Pick (Std/40/60) Sampling blade knives plus holder (10 off 0 degree blade edge)
P/N J055EZ Pick (Std/40/60) Sampling blade knives plus holder (10 off 15 degree blade edge)
P/N J056EZ Pick (Std/40/60) Sampling blade knives plus holder (10 off 30 degree blade edge)
P/N J057EZ Pick (PE) Sampling blade knives plus holder (10 off 0 degree blade edge)
P/N J058EZ Pick (PE) Sampling blade knives plus holder (10 off 15 degree blade edge)
P/N J059EZ Pick (PE) Sampling blade knives plus holder (10 off 30 degree blade edge)
P/N J060EZ Pick (Std/40/60) Diamond micro plane (Scrabbler)
P/N J061EZ Pick (PE) Diamond micro plane (Scrabbler)
P/N J062EZ Pick replacement needles (3 off - 1, 2.5 and 5 micron tips, one each)

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5. Diamond EX-Press Compression Cell

Technical information
The Diamond EX-Press is an excellent sample preparation tool for FTIR micro analysis. The diamond is a high quality Synthetic Type IIa Sumi-crystal with excellent physical and infrared transmission properties. Samples of solids and film (e.g. mineral stones, polymers and pharmaceutical materials) can be compressed to thin uniform films by using only finger tight pressure for good infrared analysis without prior sample preparation.

The cell is fitted with 1mm thick diamonds, has a working distance of only 11mm and fitted with a 3" x 2" backplate making it ideal for FTIR spectrometers without a beam condenser and Infrared Microscopes with 15X and 32X objectives.

Ordering information
P/N J010
P/N J011
Diamond EX-Press Compression Cell 1.6mm diameter clear aperture
Diamond EX-Press Compression Cell 2.0mm diameter clear aperture

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6. Fluorescent Filters and Filter Sets

Typical specification
EM = Emitter   EX = Exciter   LPD = Long Pass Dichroic
Flatness (EM): lambda/4
Parallelism (EM): 30 arc seconds
Surface finish (EM): 60-40 scratch-dig ; (EX) 80-50 scratch dig
Crossover blocking {EX/EM}: OD5 (typical)
Blocking (EX):UV-1150nm
Humidity: MIL-STD-810C, m507, p1
Temperature range: -20° C to + 80° C
Thickness (EM/EX): 4-5mm ring mounted (fits cube); LPD ~ 1mm (typical)
Available cubes for: Leica, Nikon, Olympus, Zeiss
Diameters (EM/EX): 18mm, 20mm, 22mm, 25mm
Rectangular (LPD): 26mm x 18mm, 29mmx 21mm, 36mm x 25.7mm
Available on request

: Custom Fluorescent Filters

  P/N     P/N
Set Name: Alexa Fluor 6331875   Set Name: Allophycocyanin (APC)1879
EX peak max.: 632nm615EX401876   EX peak max.: 650nm590EX551880
Dichroic645LPD1877   Dichroic625LPD1881
EM peak max.: 648nm675EM501878   EM peak max.: 660nm665EM651882
 
Set Name: Calcium Crimson1883   Set Name: Cy31887
EX peak max.: 590nm580EX201884   EX peak max.: (512) 554nm535EX501888
Dichroic595LPD1885   Dichroic566LPD1889
EM peak max.: 617nm630EM601886   EM peak max.: 570nm610EM751890
 
Set Name: Cy3 NarrowBand(NB)1891   Set Name: Cy3.51895
EX peak max.: (512) 554nm545EX301892   EX peak max.: 581nm565EX301896
Dichroic566LPD1893   Dichroic585LPD1897
EM peak max.: 570nm610EM751894   EM peak max.: 594nm620EM601898
 
Set Name: Cy51899   Set Name: Cy5.51903
EX peak max.: (650) 615nm620EX601900   EX peak max.: 675nm650EX451904
Dichroic656LPD1901   Dichroic678LPD1905
EM peak max.: 668nm700EM751902   EM peak max.: 694nm710EM501906
 
Set Name: Cy5.5 Red-Shifted 1907   Set Name: Cy71911
EX peak max.: 675nm665EX451908   EX peak max.: 743nm710EX751912
Dichroic694LPD1909   Dichroic756LPD1913
EM peak max.: 694nm725EM501910   EM peak max.: 767nm810EM901914
 
Set Name: DAPI, Hoechst, AMCA 1915   Set Name: DAPI, Hoechst, AMCA1919
EX peak max.: 359nm (NB)365EX101916   EX peak max.: 359nm360EX401920
Dichroic390LPD1917   Dichroic400LPD1921
EM peak max.: 460nm460EM501918   EM peak max.: 517nm460EM501922
 
Set Name: DsRed Narrowband Ex1923   Set Name: EGFP1927
EX peak max.: 556nm546EX121924   EX peak max.: 498nm470EX401928
Dichroic560LPD1925   Dichroic495LPD1929
EM peak max.: 584nm605EM751926   EM peak max.: 517nm525EM501930
 
Set Name: EGFP Longpass 1931   Set Name: EGFP Narrowband1935
EX peak max.: 498nm470EX401932   EX peak max.: 498nm480EX201936
Dichroic495LPD1933   Dichroic495LPD1937
EM peak max.: 517nm500EMLP1934   EM peak max.: 517nm510EM201938
 
Set Name: Eosin 1939   Set Name: Ethidium Bromide1943
EX peak max.: 525nm500EX401940   EX peak max.: 523nm535EX501944
Dichroic527LPD1941   Dichroic566LPD1945
EM peak max.: 545nm560EM401942   EM peak max.: 604nm610EM751946
 
Set Name: FITC, EGFP 1947   Set Name: FITC, PI1951
EX peak max.: 494nm480EX401948   EX peak max.: 494nm480EX801952
Dichroic505LPD1949   Dichroic510LPD1953
EM peak max.: 518nm535EM501950   EM peak max.: 520nm560EM801954
 
Set Name: FITC, EGFP Longpass1955   Set Name: GFP-Yellow1959
EX peak max.: 494nm480EX401956   EX peak max.: 498nm500EX201960
Dichroic506LPD1957   Dichroic515LPD1961
EM peak max.: 520nm510EMLP1958   EM peak max.: 517nm535EM301962
 
Set Name: wtGFP 1963   Set Name: wtGFP Longpass 1967
EX peak max.: 396nm450EX501964   EX peak max.: 396nm450EX501968
Dichroic480LPD1965   Dichroic480LPD1969
EM peak max.: 514nm510EM501966   EM peak max.: 514nm485EMLP1970
 
Set Name: Indo-Cyanine Green 1971   Set Name: Oregon Green 1975
EX peak max.: 795nm775EX501972   EX peak max.: 494nm495EX301976
Dichroic808LPD1973   Dichroic515LPD1977
EM peak max.: 815-820nm845EM551974   EM peak max.: 518nm545EM501978
 
Set Name: Phycoerythrin r&b 1979   Set Name: Piston GFP1983
EX peak max.: 565nm546EX121980   EX peak max.: 489nm470EX401984
Dichroic558LPD1981   Dichroic495LPD1985
EM peak max.: 578nm585EM401982   EM peak max.: 508nm515EM301986
 
Set Name: Propidium Iodide 1987   Set Name: Resorufin1991
EX peak max.: 536nm535EX501988   EX peak max.: 571nm577EX101992
Dichroic584LPD1989   Dichroic586LPD1993
EM peak max.: 617nm645EM751990   EM peak max.: 584nm620EM601994
 
Set Name: Rhodamine Longpass 1995   Set Name: Rhodamine-X1999
EX peak max.: 550nm545EX301996   EX peak max.: 574nm570EX202000
Dichroic570LPD1997   Dichroic585LPD2001
EM peak max.: 573nm572EMLP1998   EM peak max.: 598nm620EM602002
 
Set Name: Texas Red 2003   Set Name: TRITC 2007
EX peak max.: 595nm560EX552004   EX peak max.: 550nm535EX502008
Dichroic597LPD2005   Dichroic566LPD2009
EM peak max.: 615nm645EM752006   EM peak max.: 573nm610EM752010
 
Set Name: TRITC Red-Shift (RS)2011   Set Name: TRITC NarrowBand(NB)2015
EX peak max.: 550nm535EX502012   EX peak max.: 550nm545EX302016
Dichroic575LPD2013   Dichroic566LPD2017
EM peak max.: 573nm620EM602014   EM peak max.: 573nm610EM752018
 
Set Name: TRITC RS & NB 2019   Set Name: Kinosita TRITC2023
EX peak max.: 550nm545EX302020   EX peak max.: 550nm525EX502024
Dichroic575LPD2021   Dichroic558LPD2025
EM peak max.: 573nm620EM602022   EM peak max.: 573nm590EM502026
 

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